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产品目录

DseDI内切酶图片
产品货号:
GS2178
中文名称:
DseDI内切酶
英文名称:
DseDI
产品规格:
250U
发货周期:
1~3天
产品价格:
询价
储存条件:冷冻(-20℃)

使用说明

To obtain 100%activity,BSA should be added to the 1 x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.

技术规格

Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sitesGACNNNN↑NNGTC
来源Deinococcus species D2
Assayed onLambda DNA
Optimal bufferY(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.)+ BSA
Optimal temperature37℃
Storage conditions10 mM Tris-HCl(pH 7.5);50 mM KCl;0.1 mM EDTA;200 μg/ml BSA;7 mM 2-mercaptoethanol;50%glycerol;Store at -20℃.
LigationsAfter 5-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a.of enzyme for 16hoursat 37℃
Methlation effectMethylation sensitivity not tested
Thermal inactivationenzyme is inactivated by incubation at 80℃ for 20 mins.
Campatible EndsAasI,DrdI

相关搜索:DseDI内切酶DseDI限制性内切酶
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